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Proteins / Signaling Molecules

This product group contains both native proteins and recombinant proteins expressed and purified from bacterial, insect and mammalian expression systems. Bio-Connect also offers fusion proteins, peptides, enzymes, bacterial and viral proteins, hormones and growth factors. Furthermore, it includes chemokines and cytokines, collagenases, albumins, globulins, blood/plasma related proteins and more.

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Western blot analysis of Caspase-2 expression in HeLa cell lysate (M02384). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP2 monoclonal antibody (Catalog # M02384) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP2
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Caspase-2 Rabbit Monoclonal AntibodyM02384
Western blot analysis of Neutrophil Elastase expression in HL60 cell lysate.
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Neutrophil Elastase Rabbit Monoclonal AntibodyM01607
Western blot analysis of LIG4 expression in Ramos cell lysate using anti-LIG4 antibody (M01685). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIG4 antigen affinity purified polyclonal antibody (Catalog # M01685) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for LIG4.
Product group Proteins / Signaling Molecules
Boster Bio
Anti-DNA Ligase IV Monoclonal AntibodyM01685
Western blot analysis of CKB using anti-CKB antibody (M01695) in SHSY5Y cell lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CKB antigen affinity purified polyclonal antibody (Catalog # M01695) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Creatine kinase B type Monoclonal AntibodyM01695
Immunohistochemical analysis of paraffin-embedded human colon, using Myosin light chain kinase Antibody(M01697) MYLK was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MYLK Antibody (M01697)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Myosin light chain kinase Rabbit Monoclonal AntibodyM01697
Western blot analysis of ACSS2 expression in HepG2 cell lysate (M02809). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACSS2 monoclonal antibody (Catalog # M02809) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACSS2
Product group Proteins / Signaling Molecules
Boster Bio
Anti-ACSS2/Acetyl Coa Synthetase Rabbit Monoclonal AntibodyM02809
Western blot analysis of PAP ( prostatic acid phosphatase) expression in human prostate cancer lysate.
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Prostatic Acid Phosphatase Monoclonal AntibodyM02082-1
Western blot analysis of POR expression in A431 cell lysate using anti-POR antibody (M02166). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POR antigen affinity purified polyclonal antibody (Catalog # M02166) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for POR.
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Cytochrome P450 Reductase Monoclonal AntibodyM02166
Western blot analysis of Caspase-10 expression in Jurkat cell lysate.
Product group Proteins / Signaling Molecules
Boster Bio
Anti-Caspase-10 Rabbit Monoclonal AntibodyM02190-1
Western blot analysis of pro Caspase 10 expression in HeLa cell lysate (M02190). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP10 monoclonal antibody (Catalog # M02190) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP10
Product group Proteins / Signaling Molecules
Boster Bio
Anti-pro Caspase 10 Rabbit Monoclonal AntibodyM02190
Western blot analysis of TGM2 expression in HUVEC cell lysate.
Product group Proteins / Signaling Molecules
Boster Bio
Anti-TGM2/Transglutaminase 2 Rabbit Monoclonal AntibodyM02229-1
Western blot analysis of PIK3CD using anti-PIK3CD antibody on (1) K562 cell lysate; (2) RAW 264. cell lysate; (3) Rat kidney lysate. (M02269). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3CD antigen affinity purified polyclonal antibody (Catalog # M02269) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Proteins / Signaling Molecules
Boster Bio
Anti-PI 3 Kinase p110 delta Monoclonal AntibodyM02269
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